What is the function of DNA polymerase III *?

What is the function of DNA polymerase III *?

Summary: DNA polymerase III holoenzyme is the enzyme primarily responsible for replicative DNA synthesis in E. coli. It carries out primer-initiated 5′ to 3′ polymerization of DNA on a single-stranded DNA template, as well as 3′ to 5′ exonucleolytic editing of mispaired nucleotides.

Does DNA polymerase 3 have proofreading?

In bacteria, all three DNA polymerases (I, II and III) have the ability to proofread, using 3′ 5′ exonuclease activity. When an incorrect base pair is recognized, DNA polymerase reverses its direction by one base pair of DNA and excises the mismatched base.

How does DNA polymerase 3 proofread?

Proofreading: DNA polymerase adds a new base to the 3′ end of the growing, new strand. Polymerase detects that the bases are mispaired. Polymerase uses 3′ to 5′ exonuclease activity to remove the incorrect T from the 3′ end of the new strand.

What happens if DNA polymerase makes a mistake?

Errors during Replication. DNA replication is a highly accurate process, but mistakes can occasionally occur as when a DNA polymerase inserts a wrong base. Uncorrected mistakes may sometimes lead to serious consequences, such as cancer. Mutations: In this interactive, you can “edit” a DNA strand and cause a mutation.