How do you reconstitute primers from IDT?

How do you reconstitute primers from IDT?

To obtain a 100 µM solution, multiply # nmol x 10. That will equal the # µL to use for resuspension. For example: 20 nmol X 10 = 200 µL. IDT offers a FREE online resuspension calculator and dilution calculator to make such calculations for you.

How do you calculate primer resuspension?

To determine the amount of water to add to the lyophilized primer simply multiply the number of nmol of primer in the tube by 10. That will be the amount of water to add to make a 100 µM primer stock. For example, if there are 38.2 nmol of primer a 100 µM primer stock is created by adding 382 µl of water.

What should I resuspend primers in?

We recommend resuspending oligos in a TE buffer solution, such as IDTE, to maintain a constant pH that supports oligo stability (IDTE is available from IDT at pH 7.5 and pH 8.0). Alternatively, resuspend oligos in nuclease-free, sterile water, pH 7.0 (HPLC-grade is preferable; available from IDT).

What temperature should primers be stored at?

After diultion, you should store primers at -20C. lyophilized primers can be transported at normal temperatures, we usually purchase our primers from europe. Best regards. Primers, when suspended, should be stored in a freezer at -20C.

How do you reconstitute primers Sigma?

Follow these steps:

1. Resuspend the oligonucleotide in 400 µL of water or buffer.
2. Dilute 12 µL into 988 µL of sterile, nuclease-free water.
3. Take an A260 reading of the 1 mL sample in a cuvette.
4. Ensure the OD value is in the linear range (~0.1 to 1 OD).
5. Multiply the OD of the sample volume by the dilution factor.

Can you vortex primers?

Don’t vortex too much. But you can vortex gently for 5-10 for proper mixing. It would not break primers.

Are primers OK at room temperature?

DNA is usually considered stable at room temperature for about 2 weeks, 4 °C for about 2 months, -20 °C for about 2 years and -80 °C for >10 years. It is recommended that you store lyophilized primers at fridge or freezer temperatures anyways.

How long can primers stay at room temperature?

Condition	Duration
Room temperature	Stable for approximately 3 – 6 months, whether dry or in TE buffer
Hot day (non-temperature-controlled shipping or storage in warehouse)	Stable for approximately 1 – 2 months, whether dry or in TE buffer