How does DNA replication in eukaryotes?
Replication processes permit the copying of a single DNA double helix into two DNA helices, which are divided into the daughter cells at mitosis. In eukaryotes, the vast majority of DNA synthesis occurs during S phase of the cell cycle, and the entire genome must be unwound and duplicated to form two daughter copies.
What is the role of DNA polymerase III in DNA replication?
The main function of the third polymerase, Pol III, is duplication of the chromosomal DNA, while other DNA polymerases are involved mostly in DNA repair and translesion DNA synthesis. Together with a DNA helicase and a primase, Pol III HE participates in the replicative apparatus that acts at the replication fork.
What is the function of polymerase III?
Summary: DNA polymerase III holoenzyme is the enzyme primarily responsible for replicative DNA synthesis in E. coli. It carries out primer-initiated 5′ to 3′ polymerization of DNA on a single-stranded DNA template, as well as 3′ to 5′ exonucleolytic editing of mispaired nucleotides.
What is needed for DNA replication?
New DNA is made by enzymes called DNA polymerases, which require a template and a primer (starter) and synthesize DNA in the 5′ to 3′ direction. DNA replication requires other enzymes in addition to DNA polymerase, including DNA primase, DNA helicase, DNA ligase, and topoisomerase.