How does the CyQUANT assay work?
The CyQUANT Cell Proliferation Assay Kit from Life Technologies is a sensitive, rapid and convenient way to quantitate cell growth using a fluorescence microplate reader. CyQUANT GR dye binds to cellular nucleic acids, allowing cell numbers to be calculated from a standard curve.
How do I check cell proliferation?
There are several methods available to measure cell proliferation rates. One method is to measure the overall metabolic activity inside a cell. Several dyes are available that can permeabilize a cell and react with certain enzymes and other factors and form a colored end-product which can be easily detected.
How can you measure cell proliferation with flow cytometry?
Approaches to assess cell proliferation by flow cytometry include: measuring DNA synthesis with Invitrogen Click-iT EdU Flow Cytometry Assay Kits or traditional bromodeoxyuridine (BrdU) staining, tracking generations of cell division with Invitrogen CellTrace Cell Proliferation Kits or carboxyfluorescein succinimidyl …
What does CyQUANT measure?
The CyQUANT® Cell Proliferation Assay is a highly sensitive fluorescence-based method for quantifying cells and assessing cell proliferation and cytotoxicity.
What is cell proliferation assay?
Cell proliferation assays typically detect changes in the number of cells in a division or changes in a cell population. Cell proliferation assays are mainly divided into four methods: metabolic activity assays, cell proliferation marker assays, ATP concentration assays, and DNA synthesis assays.
What is cell death assay?
Cell viability and cytotoxicity assays measure cellular or metabolic changes associated with viable or nonviable cells. These assays can detect structural changes such as loss of membrane integrity upon cell death or physiological and biochemical activities indicative of living cells.
How does BrdU staining work?
Bromodeoxyuridine (BrdU) is a thymidine analog that incorporates DNA of dividing cells during the S-phase of the cell cycle. Once incorporated, these nucleoside analogs serve as cell cycle and proliferation markers that can be detected using labeled probes to identify cells that are actively proliferating.
What is MTS cell proliferation?
The MTS Cell Proliferation Assay Kit is a colorimetric method for sensitive quantification of viable cells in proliferation and cytotoxicity assay. The method is based on the reduction of MTS tetrazolium compound by viable cells to generate a colored formazan product that is soluble in cell culture media.
What are cyquant cell proliferation assays kits?
Rapid and easy to use, CyQUANT Cell Proliferation Assays Kits require no washes, extractions, growth medium changes, or long incubations, and don’t rely metabolic status of the cell. See your assay options with our Selection Guide.
What is the cyquant® kit?
The basis for the CyQUANT® kit is the use of a proprietary green fluorescent dye, CyQUANT® GR dye, which exhibits strong fluorescence enhancement when bound to cellular nucleic acids. Frozen cells are simply thawed and lysed by addition of a buffer containing the CyQUANT® GR dye; fluorescence is then measured directly.
What is the difference between colorimetric-based and cyquant-based assays?
CyQUANT Cell Proliferation Assays are ideal for high-throughput screening, are more sensitive than colorimetric-based assays, and are not radioactive. Rapid and easy to use, CyQUANT Cell Proliferation Assays Kits require no washes, extractions, growth medium changes, or long incubations, and don’t rely metabolic status of the cell.
How do I store my cells for my cyquant® assay?
Freeze the cells in the microplate and store at -70°C until samples are to be assayed. Note: Frozen cells in microplates and cell pellets in centrifuge tubes can be stored at -70°C for up to four weeks. The freezing step is important for efficient cell lysis in the CyQUANT® assay.