How long does PBMCs last?
When a PVC bag is used for freezing and storage of PBMCs at -80 degrees C, the duration of frozen storage should not exceed 1.5 years, whereas PBMCs frozen in a polyolefin bag can be stored in a -135 degrees C freezer for as long as 2.4 years.
How PBMC remove platelets?
For removal of platelets, resuspend the cell pellet in 50 mL of buffer and centrifuge at 200×g for 10–15 minutes at 20 °C. Carefully remove the supernatant completely. ▲ Note: This step will increase the purity of the target cells in the subsequent MACS® Cell Separation.
What is SepMate?
SepMate™ is an innovative tube that enables consistent and hassle-free peripheral blood mononuclear cell (PBMC) isolation in just 15 minutes. The SepMate™ insert allows users to quickly layer blood over the density gradient medium, and prevents the layers from mixing.
How does histopaque separate blood?
Anticoagulated blood is layered onto Histopaque-1077. During centrifugation, erythrocytes are aggregated by polysucrose and rapidly sediment. Granulocytes become slightly hypertonic, which increases their sedimentation rate, resulting in pelleting at the bottom of the centrifuge tube.
What is PBMC used for?
PBMCs are widely used in research and toxicology applications. Peripheral blood mononuclear cells (PBMC) give selective responses to the immune system and are the major cells in the human body immunity.
What is PBMC?
Peripheral blood mononuclear cells (PBMC) give selective responses to the immune system and are the major cells in the human body immunity. They contain several types of cells such as lymphocytes,monocytes or macrophages.
How is sepsepmate™ manufactured?
SepMate™ is manufactured following current Good Manufacturing Practices (cGMP) and tubes are sterilized by irradiation. SepMate™ is registered as an In Vitro Diagnostic (IVD) device in Australia, Canada, Europe and the United States for the isolation of mononuclear cells from whole blood or bone marrow by density gradient centrifugation.
What is SepMate™ PBMC isolation?
The typical PBMC isolation protocol is laborious, requiring slow layering of blood on density gradient medium (e.g. Lymphoprep™, Ficoll-Paque™), lengthy 30-minute centrifugation with the brake off, and careful harvest of PBMCs using a pipette. SepMate™ simplifies and speeds up the PBMC isolation protocol to an easy 15-minute procedure.
Can SepMate™ be used as laboratory equipment?
In China SepMate™ is considered a non-medical device by the China Food and Drug Administration (CFDA), and should therefore be used as general laboratory equipment. The end user is responsible for determining whether the product is suitable for their specific application.
What is RosetteSep™ SepMate?
SepMate™ is the ideal solution for laboratories having high rates of blood samples per day. It lowers the cost by reducing the tech time. To isolate purified cell subsets from whole blood, simply incubate your sample with the appropriate RosetteSep™ cocktail for 10 minutes prior to pipetting onto density medium in a SepMate™ tube.