How many wells are in a PCR plate?
384
PCR Plate, 384-well, standard.
What is a PCR plate?
BRAND PCR plates are manufactured from high-quality virgin plastics for excellent, reproducible results in most PCR applications. BRAND PCR plates are free of DNA, DNase, RNase, and other PCR inhibitors. BRAND PCR plates have thin, uniform walls to ensure consistent, precise thermal transfer and excellent results.
What are PCR plates used for?
With common well matrixes and high or low profile designs that are compatible with thermal cyclers, the PCR plates facilitate rapid segment amplification when manual processing is not wanted. The PCR plate has seal integrity from –80 to –200°C, which is highly effective in heat sealing and reduces heat transfer.
What is a low profile PCR plate?
The low profile PCR plate has shorter wells than the standard profile plate (AB-0600), decreasing the dead space between the heated lid of the thermal cycler and the sample. This eliminates condensation on the side wall of the tube, preventing reduction in PCR volume and increasing the efficiency of the reaction.
What is the volume of a 96-well plate?
CulturPlate Microplates
| Well format | 24-well | 96-well |
|---|---|---|
| #columns | 6 | 12 |
| Well volume | 2.39 mL | 400 µL |
| Recommended working volume | 0.5-2.39 mL | 80 µL-350 µL |
| Height (mm) | 18.70 | 14.60 |
What is the function of well plate?
The well plate has become a standard tool in analytical research and clinical diagnostic testing laboratories¹. ELISA is very common and is the basis of most modern medical diagnostic testing in humans and animals. The wells of the microplate typically hold between tens of nanoliters to several milliliters of liquid.
What is PCR cabinet?
Polymerase Chain Reaction (PCR) cabinet provides HEPA-filtered vertical laminar flow to purge the work area of contaminants between amplifications and during preparatory procedures. Under these circumstances, PCR Cabinets are used for reagent preparation and sample preparation to minimize contamination.
What are low profile plates?
Low-profile (around 15.5 mm) wells are the newer design that reduces the potential for condensate formation and offers advantages for light capture in fluorescence assays, low-volume reactions, and fast PCR.
What are the different types of 96-well PCR plates?
High-profile 96-Well PCR Plates are available in unskirted and semi-skirted formats. Hard-Shell 96-Well Semi-Skirted PCR Plates, with either clear or white wells, are ideal for robotic handling.
How much volume does a 96 well plate hold?
Description The Thermo Scientific 96-Well Non-Skirted Plates for use in PCR and qPCR applications hold up to 0.3 mL of well volume when paired with adhesive films or foils. The plates feature a cut corner located at H1 and are compatible with most thermal cyclers. Features of Thermo Scientific 96-Well Non-Skirted Plates
What is the best type of PCR plate for robotic handling?
Hard-Shell 96-Well Semi-Skirted PCR Plates, with either clear or white wells, are ideal for robotic handling. Low-profile 96-Well PCR Plates in a choice of skirt formats; barcoding; clear, white, or black wells; and colored plates, including Hard-Shell ® 96-Well PCR Plates optimized for the Roche LightCycler 480 system.
Why choose Thermo Scientific PCR plastics?
All Thermo Scientific PCR plastics are certified free from RNase, DNase and human DNA. In contrast, during typical non-clean room production, plastics are exposed to many contaminants including dust, bacterial cells, and DNA.