What are E0771 cells?
The E0771 cell line is a spontaneously developing medullary breast adenocarcinoma from C57BL/6 mice.[1] Parental E0771 is poorly metastatic when compared to 4T1[2] and has homozygous mutations in the Trp53 and KRAS genes.[3]
Is E0771 triple negative?
Despite their isolation in the late 1940s, characterization of hormonal receptors and classification of EO771 line remains controversial. Indeed, its classification diverges according to the authors, which is considered as triple negative in 10 publications6, 12, 16-23 and as ERα+ in 19 publications.
What is MMTV PyMT?
The MMTV-PyMT (mouse mammary tumor virus-polyoma middle tumor-antigen) mouse model of breast cancer is widely used and well characterized. MMTV-PyMT mice develop spontaneous mammary tumors that closely resemble the progression and morphology of human breast cancers [12-14].
Is 4T1 triple-negative?
4T1 is a triple-negative mouse breast cancer cell line. Pharmatest utilises a 4T1 cell line with stable expression of green fluorescent protein (GFP) enabling easy and efficient detection of whole-body tumour burden. Use of immunocompetent mice in 4T1 models allows efficacy testing of immonomodulatory compounds.
What is B16F10?
B16F10 (ATCC® CCL-6475™) is a murine melanoma cell line from a C57BL/6J mouse. It is a subclone of the B16 tumor line, generated by injecting mice with B16 tumor cells, collecting and culturing secondary tumor growths, and injecting them into fresh mice, a total of 10 times.
Is TNBC a 4T1?
The 4T1 cell line represents TNBC, and over 1,000 studies have reported on the 4T1 cell line model. The mammary intraductal (MIND) model, in which cells are injected into the mouse milk duct system, can accurately simulate the microenvironment of breast tumors31,32,33,34.
Do MCF-7 cells express HER2?
The MCF7 breast cancer cell line was transfected with a pcDNA 3.1 construct encoding human HER2 that was derived from a retroviral construct (Riese et al, 1995), and HER2 expressing clones were identified by immunoblot analysis (Figure 1A).
Are MCF-7 cells HER2 positive?
MCF-7 breast cancer cells stably overexpressing ERbeta1 exhibited elevated >5-fold HER2 mRNA levels and elevated >3-fold HER2 protein levels even in the absence of estradiol.
What is e0771 breast cancer cell line?
E0771 murine breast cancer cell line adenocarcinoma cell line originally isolated as a spontaneous tumor from C57BL/6 mouse (Sugiura, K and Stock, C.C., 1952) and adapted for anti‐cancer drug testing by Sirotnak et al., 1984 Re-packaging or commercial use of this product requires licensing approval.
What pathogens does seselected lots of the e0771 cell line screen for?
Selected Lots of the E0771 cell line are authenticated by short tandem repeat profiling and screened for the following pathogens: In addition, species-specific PCR evaluation is performed to rule out any contamination by rat, human, hamster or monkey cells. Screening for additional pathogens may be available upon request.
Are eo771 cells triple negative or ERα + ?
Nevertheless, this type of mammary tumour remains poorly characterized and the results are divergent concerning its classification. To date, among the 100 publications studying EO771 cells, 10 articles consider them as triple negative and 19 as ERα + .
What is the density of eto771 cells?
EO771 cells were plated at a density of 4.5 × 10 5 cells in 75 cm 2 flasks in a complete medium. Cultures were at 37 °C in a humidified atmosphere with 5% CO 2. After 48 h, they were treated by the tamoxifen IC50 (14 µM) previously determined.