What direction does replication always occurs in?
To replicate DNA and RNA nucleotide chains, new copies are synthesized from existing ones. This copying process always happens in a “forward” direction, from the 5′ to the 3′ end. During the process the double-stranded DNA is separated into two strands and aligned in opposite directions, complicating the matter.
Why are Okazaki fragments made on the lagging strand?
Function: A building block for DNA synthesis of the lagging strand. On one template strand, DNA polymerase synthesizes new DNA in a direction away from the replication fork movement. Because of this, the new DNA synthesized on that template is made in a discontinuous fashion; each segment is called an Okazaki fragment.
What direction are Okazaki fragments synthesized?
Okazaki Fragments. At a replication fork, both strands are synthesized in a 5′ → 3′ direction. The leading strand is synthesized continuously, whereas the lagging strand is synthesized in short pieces termed Okazaki fragments.
Are Okazaki fragments generated?
Leading and Lagging Strands Okazaki fragments are initiated by creation of a new RNA primer by the primosome. To restart DNA synthesis, the DNA clamp loader releases the lagging strand from the sliding clamp, and then reattaches the clamp at the new RNA primer.
What determines the length of Okazaki fragments?
Although the prokaryotic fragments are ∼1200 nucleotides long, the eukaryotic fragments are much shorter, with lengths determined by nucleosome periodicity.
What removes Okazaki fragments?
Flap endonuclease 1 (FEN1) is responsible for processing Okazaki fragments. It works with DNA polymerase to remove the RNA primer of an Okazaki fragment and can remove the 5′ ribonucleotide and 5′ flaps when DNA polymerase displaces the strands during lagging strand synthesis.
What enzyme joins Okazaki fragments quizlet?
Ligase assembles nucleotides into Okazaki fragments. Then polymerase joins these fragments together into a DNA strand.
Which of the following builds new strands of DNA quizlet?
DNA polymerase is an enzyme that builds new strands of DNA. It does this by adding DNA nucleotides one at a time.
What enzyme removes the RNA primers?
Which enzyme joins the DNA segments?