What does AmpliTaq Gold DNA polymerase do?
Due to its purity, AmpliTaq Gold 360 DNA Polymerase efficiently amplifies low-copy targets, making it suitable for detection of sequences that are rare or degraded. AmpliTaq Gold 360 DNA Polymerase is able to amplify sequences up to 5 kb.
What is AmpliTaq?
AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli.
What does Hot Start PCR do?
Hot Start PCR allows for reaction set up at room temperature without non-specific amplification and primer dimer formation. Whereas conventional PCR is often utilized to make exponential copies of your DNA target sequence without an additional temperature-sensitive reaction activation component.
Does Taq polymerase require a primer?
PCR primers Like other DNA polymerases, Taq polymerase can only make DNA if it’s given a primer, a short sequence of nucleotides that provides a starting point for DNA synthesis.
How does a hot start work?
The hot start simply adds more air to the fuel/air mix needed to start a HOT or STALLED engine.
Is Taq polymerase still used?
Taq polymerase remains to be the most ubiquitously used polymerase for PCR. This enzyme has a high rate of dNTP incorporation but does not have any proof-reading activity. This means that Taq is prone to introducing base-pair errors during PCR amplification.
What is a hot start kit?
You’ve probably seen them around – a kit thrown together and called a “hot start kit”, promising to remedy these issues on your modified classic or muscle car. The kits claim to eliminate slow cranking due to engine heat and delivery and offer an extra 12V of cranking power, but we recommend you avoid these kits.
What is amplitaq gold?
AmpliTaq Gold is a thermostable DNA polymerase designed to make advanced Poly- merase Chain Reaction (“PCR”) techniques easy. With AmpliTaq Gold, Hot Start PCR and Time Release PCR (see below) can be introduced into existing amplification sys- tems with modification of cycling parameters or reaction conditions.
What is amplitaq gold® DNA polymerase?
AmpliTaq Gold® DNA Polymerase is a chemically modified form of AmpliTaq® DNA Polymerase requiring thermal activation. Features of this enzyme: The modified enzyme is provided in an inactive state. Upon thermal activation, the modifier is permanently released, regenerating active enzyme.
How does the enhanced 360 Gold buffer work?
When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates. Also included with this product is a 360 GC Enhancer to work through challenging GC-rich templates.