What is 6X loading dye?
Thermo Scientific 6X DNA Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains two different dyes (bromophenol blue and xylene cyanol FF) for visual tracking of DNA migration during electrophoresis.
How much of 6X loading dye Do we need to add 10ul of our PCR product?
Add 2 µl of UView 6x loading dye to each 10 µl sample of DNA.
What volume is 6X loading dye?
5 µl
Use 5 µl of Gel Loading Dye, Blue (6X) per 25 µl reaction, or 10 µl per 50 µl reaction. Mix well before loading gel.
How do you make 6X DNA Loading dye?
Preparation: 6x Loading Dye Solution deionised water at a ratio 1:1:4 for example, 5 µl 1 kb ladder : 5 µl 6x loading dye : 20 µl water. By applying 30.0 µl of this mixture, you’ll have 1.0 µg of total DNA per lane.
How do you dilute 6X DNA loading dye?
6X Universal & Glow Dyes Dilute one part 6X Dye solution into five parts of sample solution to give a final concentration of 1X Dye solution. The sample is then ready to load to a gel. For Example: 10µl sample and 2µl 6X Dye Solution.
Is loading dye the same as tracking dye?
DNA loading dyes normally contain: EDTA, which binds divalent metal ions that may interfere with electrophoresis. Tracking dyes (bromophenol blue, xylene cyanol FF, or orange G), which help monitor the progress of electrophoresis by the migration of the dyes.
How do you make 6X bromophenol blue?
Step 1: To prepare 10 ml of 6X DNA loading dye, weigh out 25 mg bromophenol blue, 25 mg xylene cyanol FF, and 4 g Ficoll 400. Transfer them to a screw-capped tube (graduated polypropylene centrifuge tube). Add 7 ml deionized / Milli-Q water. Mix until all ingredients dissolve completely.
What is 6X loading buffer?
6X B/Loading Buffer is used as a loading dye for visual tracking of DNA migration during electrophoresis. It incorporates Bromophenol blue. Bromophenol blue migrates fast in the agarose gel and corresponds to the migration of a 300 – 500 bp long DNA fragment in a 1% agarose gel.