What is CellTiter Glo?
The CellTiter-Glo® Luminescent Cell Viability Assay is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, an indicator of metabolically active cells. The amount of ATP is directly proportional to the number of cells present in culture.
How do I use Glo cell titer?
Add a volume of CellTiter-Glo® Reagent equal to the volume of cell culture medium present in each well (e.g., add 100µl of reagent to 100µl of medium containing cells for a 96-well plate, or add 25µl of reagent to 25µl of medium containing cells for a 384-well plate).
What cellular chemical does the CellTiter Glo assay quantify?
The CellTiter-Glo® 2.0 Assay determines the number of viable cells in culture by quantifying ATP, which indicates the presence of metabolically active cells.
Is MTT toxic to cells?
Toxicity. MTT has been reported to be toxic to eukaryotic cells (10). Exposure of cells to MTT resulted in dramatic changes in morphology during the formation of formazan crystals (Figure 1). The MTT assay uses reducing equivalents such as the co-enzyme NADH to convert MTT into a colored formazan product.
How long is cell titer glo signal stable?
The luminescent signal generated by the CellTiter Glo® Assay is stable. The protocol claims a half-life more than 5 hours. I have found this to be true. I have read the plate at 10 minutes, 30 minutes and 90 minutes.
What is proliferation of a cell?
Listen to pronunciation. (sel proh-LIH-feh-RAY-shun) An increase in the number of cells as a result of cell growth and cell division.
What is MTT used for?
Introduction. The MTT assay is used to measure cellular metabolic activity as an indicator of cell viability, proliferation and cytotoxicity.
What does celltiter Glo measure?
CellTiter-Glo® 2.0 Measures ATP, a Key Indicator of Cell Health. CellTiter-Glo® 2.0, based on the original CellTiter-Glo® Cell Viability Assay chemistry, is a single-reagent assay with improved stability for convenient storage and simpler setup.
Should I shake the plate after adding celltiter-Glo® reagent?
Suspension cell lines (e.g., Jurkat cells) generally require less mixing to achieve lysis and extract ATP than adherent cells (e.g., L929 cells). Tests were done to evaluate the effect of shaking the plate after adding the CellTiter-Glo® Reagent.
What is the celltiter 96 ® aqueous one solution cell proliferation assay (MTS)?
When using the CellTiter 96 ® AQueous One Solution Cell Proliferation Assay (MTS) (Cat.# G3582), negatively charged compounds must be combined with intermediate electron coupling reagents, which can enter cells, be reduced and then exit the cell to convert tetrazolium to the soluble formazan product.
How does the celltiter-Fluor™ assay detect viable cells?
Figure 3. The CellTiter-Fluor™ Assay detects protease activity in living cells. Tetrazolium compounds used to detect viable cells fall into two basic categories: Viable cells with active metabolism are able to convert MTT into a purple-colored formazan product. Thus, color formation can be a useful marker of viable cells.