What is FACSCalibur flow cytometer?
The Becton Dickinson FACSCalibur Flow Cytometer offers a unique approach to flow cytometry, allowing users to perform both cell analysis and cell sorting in a single system, providing flexibility for a variety of research and clinical applications.
What are the 2 lasers included in the 4 colors BD FACSCalibur?
The BD FACSCalibur dual-laser design provides the flexibility and sensitivity needed for multicolor analysis. Two lasers, an air-cooled argon laser and a red diode laser, are spatially separated for high sensitivity, minimal compensation, and maximum flexibility in fluorochrome selection.
What is the principle of flow cytometry?
Flow cytometry (FCM) is a technique which enables rapid analysis of statistically significant number of cells at single cell level. The main principle of this technique is based on scattering of light and emission of fluorescence which occur when a laser beam hits the cells moving in a directed fluid stream.
What is a FACSCalibur?
The FACSCalibur standard instrument configuration is a five-detector flow cytometer that consists of fluidic, optical, and electronic systems, and a built-in, air-cooled, argon-ion laser. The FACSCalibur system consists of a sensor unit, the FACStation data management system, and various software packages.
What is the difference between Elisa and flow cytometry?
The difference between ELISA and flow cytometry is usually below zero point (Figure 1B). However, it was observed that as the mean values increased, the difference steadily decreased in general. The IL-1β levels were measured by flow cytometry as 0−2% more than the ELISA method at lower values of IL-1β.
How long does a flow cytometry test take?
The test takes approximately three hours and consists of staining the cells, acquiring the cells on a flow cytometer, and then having a skilled technologist analyze the results that have been saved to a computer file.
Is flow cytometry an immunoassay?
Flow Cytometric Microsphere-Based Immunoassay: Analysis of Secreted Cytokines in Whole-Blood Samples from Asthmatics.
What are CD4 markers?
Also known as T4, and Leu-3, CD4 is a 55 kDa type I transmembrane glycoprotein and member of the immunoglobulin superfamily. Primarily described as a marker for T cell subsets it can also be found on NKT cells, innate lymphoid cells and macrophages.