What is glucose oxidase made of?
The glucose oxidase enzyme is commercially produced from Aspergillus niger and Penicillium glaucum through a solid-state fermentation method. Muller was first to report the catalyzation of glucose oxidase and the breakdown of glucose into gluconic acid in the presence of dissolved oxygen (Muller, 1928).
What is glucose oxidase method?
The enzyme glucose oxidase reacts with glucose, water, and oxygen to form gluconic acid and hydrogen peroxide. The hydrogen peroxide can then be used to oxidize a chromogen or the consumption of oxygen measured to estimate the amount of glucose present.
What is the molecular weight of glucose oxidase?
Glucose oxidase from Aspergillus niger is a dimer consisting of 2 equal subunits with a molecular mass of 80 kDa each. Each subunit contains one flavin adenine dinulceotide moiety and one iron.
What is the principle of glucose oxidase?
Principle: Glucose oxidase is an enzyme extracted from the growth medium of Aspergillus niger. Glucose oxidase catalyse the oxidation of Beta D- glucose present in the plasma to D glucono -1 ,5 – lactone with the formation of hydrogen peroxide; the lactone is then slowly hydrolysed to D-gluconic acid.
How is glucose oxidase manufactured?
Glucose oxidase is also produced by the hypopharyngeal glands of honeybee workers and deposited into honey where it acts as a natural preservative. GOx at the surface of the honey reduces atmospheric O2 to hydrogen peroxide (H2O2), which acts as an antimicrobial barrier.
What type of compound is glucose oxidase?
Glucose oxidase (GOx) used as biocatalytic anodes is an FAD (flavin adenine dinucleotide) enzyme, which very specifically oxidises β-D-glucose, reducing dioxygen to hydrogen peroxide in the process.
Why does glucose oxidase only react with glucose?
The glucose oxidase binds specifically to β-D-glucopyranose and does not act on α-D-glucose. It oxidises all of the glucose in solution because the equilibrium between the α and β anomers is driven towards the β side as it is consumed in the reaction.
What is the substrate of glucose oxidase?
A simple and inexpensive glucose oxidase substrate system was developed for enzyme immunoassay. This system is based on the formation of a coloured complex between polyvinyl alcohol or starch and iodine produced from iodide in the presence of hydrogen peroxide generated by the glucose oxidase reaction.
What type of enzyme is glucose oxidase?
Glucose oxidase is a subset of oxidoreductase enzymes that catalyzes the transfer of electrons from an oxidant to a reductant. Glucose oxidases use oxygen as an external electron acceptor that releases hydrogen peroxide (H2 O2 ).
Is glucose oxidase selective against galactose?
However, glucose sensors using glucose dehydrogenases (GDH) and pyrroloquinoline quinone (PQQ) dehydrogenases for direct electro-oxidation of glucose lack specificity and are susceptible to interference from other sugars in the body such as lactose, galactose, maltose, cellulose, and xylose.
Is glucose oxidase A reagent?
Glucose oxidase/peroxidase reagent along with O-Dianisidine was used for the determination of blood and urinary glucose, especially the presence of fructose for both research and clinical purpose. It may be used for determining glucose in plasma or serum.
Does glucose oxidase react with galactose?
The removal of the blood glucose by yeast fermentation in a conventional blood galactose method is replaced by an enzymic reaction using a glucose oxidase-catalase reagent. The method can be readily modified to suit a variety of blood sugar estimation methods.