What is miRNA microarray?
MicroRNAs (miRNAs) are small, noncoding RNAs that regulate the expression of target mRNAs. Microarray-based expression analysis is an ideal strategy for identifying candidate miRNAs that correlate with biological pathways and for generating molecular signatures of disease states.
What does miRNA do to mRNA?
miRNAs (microRNAs) are short non-coding RNAs that regulate gene expression post-transcriptionally. They generally bind to the 3′-UTR (untranslated region) of their target mRNAs and repress protein production by destabilizing the mRNA and translational silencing.
Can a microarray detect miRNA?
The detection of miRNA expression is a very important first step in miRNA exploration. Several methodologies, including cloning, northern blotting, real-time RT-PCR, microRNA arrays and ISH (in situ hybridization), have been developed and applied successfully in miRNA profiling.
Why is miRNA important?
miRNAs have key roles in the regulation of distinct processes in mammals. They provide a key and powerful tool in gene regulation and thus a potential novel class of therapeutic targets. miRNAs play an evolutionarily conserved developmental role and diverse physiological functions in animal.
What is the role of microRNA?
MicroRNAs (miRNAs) are a class of non-coding RNAs that play important roles in regulating gene expression. The majority of miRNAs are transcribed from DNA sequences into primary miRNAs and processed into precursor miRNAs, and finally mature miRNAs.
What does SNP array test for?
The SNP array test looks for changes in specific areas of a person’s chromosomes, such as gains (duplications) or losses (deletions). These gains or losses result in extra or missing copies of genetic material.
What is a microarray used for?
The DNA microarray is a tool used to determine whether the DNA from a particular individual contains a mutation in genes.
What is micmicron™ miRNA agomir?
micrON™ miRNA agomir is a specially chemically modified miRNA agonist that functions by mimicking endogenous miRNAs into the miRISC complex to regulate the expression of target gene mRNA.
What is microRNA agomir/ antagomir synthesis service?
MicroRNA Agomir/ Antagomir Synthesis Service. Creative Biogene provides agomir/ antagomir synthesis service that covers all human, mouse and rat miRNAs in the current miRBase. They are synthetic RNA molecules developed for in vivo miRNA functional studies.
What is the recommended concentration range of Mirna agomir/antagomir?
We suggest that the concentration range of miRNA Agomir is 1-100 nM and miRNA Antagomir is 15-100nM. The recommended amount of transfection reagent should be adjusted appropriately according to the reagent you ordered; The added amount shown is the final concentration of miRNA Agomir/Antagomir at 30 nM.
What is agomir used for?
Agomir is chemically-modified double-strand miRNA mimic with modified antisense strand: 2 phosphorothioates at the 5′ end, 4 phosphorothioates at the 3′ end, 3′ end cholesterol group, and full-length nucleotide 2′-methoxy modification. Agomirs behave like mature endogenous miRNA and are used for gain-of-function studies.