What is pooled sample testing?
See full answerPooling samples involves mixing several samples together in a “batch” or pooled sample, then testing the pooled sample with a diagnostic test. This approach increases the number of individuals that can be tested using the same amount of resources. For example, four samples may be tested together, using only the resources needed for a single test. However, because samples are diluted, which could result in less viral genetic material available to detect, there is a greater likelihood of false negative results, particularly if not properly validated. This method of pooling samples works well when there is a low prevalence of cases, meaning more negative results are expected than positive results.
What is pooling testing for COVID-19?
Pooling—sometimes referred to as pool testing or pooled testing—means combining the same type of specimen from several people and conducting one NAAT laboratory test on the combined pool of specimens to detect SARS-CoV-2, the virus that causes COVID-19. Pooled tests that return positive results will require each specimen in the pool to be retested individually to determine which individual(s) are positive.
What precautions should I take if I have been in close contact with someone who has COVID-19?
Stay home for 14 days after your last contact with a person who has COVID-19.
What are the advantages of pooling test results?
Pooling allows laboratories to test more samples with fewer testing materials. It could be useful in scenarios like returning groups of workers to a workplace. What happens if the pooled test result is negative? If a pooled test result is negative, then all the samples can be presumed negative with the single test.
What should I do if the pooled test result is positive?
If the pooled test result is positive, each of the specimens in the pool will need to be tested individually to determine which specimen (s) is (are) positive. Sufficient volume of the specimen must be available for any subsequent individual retesting.
What happens if my pool test returns a positive result?
Pooled tests that return positive results will require each specimen in the pool to be retested individually to determine which individual (s) are positive.
What is the FDA’s recommendation for pooling testing?
Generally, the FDA recommends validating the test with either pooling approach in a way that preserves the sensitivity of the test as much as possible. That is, it is preferable to use an approach where all specimens identified as positive when tested individually are also identified as positive when tested using the pooled testing approach.