What is proof reading and DNA repairing?
During DNA synthesis, most DNA polymerases “check their work,” fixing the majority of mispaired bases in a process called proofreading. Immediately after DNA synthesis, any remaining mispaired bases can be detected and replaced in a process called mismatch repair.
Where is exonuclease found?
One of the most important routes of RNA degradation in both archaea and eukaryotes is by the multi-protein exosome, which consists of multiple exoribonucleases. Besides, exonucleases are also found in the venoms of snakes and lizards.
What’s exonuclease activity?
Terminology: The ability to remove nucleotides one at a time from the end of a chain is called exonuclease activity. (exo = from the exterior or end).
Which direction are Okazaki fragments synthesized?
Okazaki Fragments. At a replication fork, both strands are synthesized in a 5′ → 3′ direction. The leading strand is synthesized continuously, whereas the lagging strand is synthesized in short pieces termed Okazaki fragments.
What is the direction of the lagging strand?
This is the parent strand of DNA which runs in the 3′ to 5′ direction toward the fork, and it’s able to be replicated continuously by DNA polymerase. The other strand is called the lagging strand. This is the parent strand which runs in the 5′ to 3′ direction toward the fork, and it’s replicated discontinuously.