What is the effect of temperature on enzyme activity?
The effect of Temperature on Enzyme. •The rate of an enzyme catalyzed reaction is affected by changes in temperature •Each enzyme has a temperature that it works optimally in called (Optimum Temperature) •For most enzymes, the optimum temperature is at or above the temperature of the cells in which the enzyme is found in vivo.
What are enzymes and how do they work?
Enzymes are catalysts to specific biological reactions, and are influenced by temperature just the same. In this lab, you will measure enzyme activity in a solution and measure the effect of five different temperatures: 30°C, 40°C, 50°C, 60°C, and 70°C.
How does temperature affect the activity of amylase?
At low temperatures the amylase will break starch down slowly due to reduced kinetic energy. At high temperatures the amylase will break starch down slowly or not at all due to denaturation of the enzyme’s active site. Same volume and concentration of starch.
What can I do to improve my enzyme experiment results?
Evaluate your experimental apparatus and methods, commenting on the accuracy and precision of your results. Propose any changes to the procedure that would improve the quality of the results. Suggest further experimental work you could carry out to extend your understanding of enzyme activity and temperature.
How do enzymes work?
Background: Enzymes are biological catalysts that speed up the rates of chemical reactions that take place within cells. In this investigation, you will study several factors that affect the activity of enzymes.
What happens to the glucose count as the temperature increases?
Although the glucose count continued to grow as the temperature increased, at the highest temperature, 65°C, no glucose was present in the test tube. This happens because after the lactase enzyme is heated up to a certain temperature the enzyme starts to denature.
What is the temperature at which glucose is denatured?
At the lowest temperature little glucose was present because the reaction rate decreases as the temperature lowers. At the highest temperature, 65°C, the glucose strip showed a negative result because the enzyme had denatured.