What is toluidine blue stain used for?
Toluidine blue has been used in vivo to identify dysplasia and carcinoma of the oral cavity. Use of toluidine blue in tissue sections is done with the aim to highlight components, such as mast cells granules, mucins, and cartilage.
How do you make toluidine blue stain?
Toluidine Blue Working Solution (pH 2.0~2.5): Toluidine blue stock solution ————– 5 ml 1% Sodium chloride, pH 2.3 ————— 45 ml Mix well. The pH should be around 2.3 and less than 2.5 Make this solution fresh and discard after use. pH higher than 2.5 will make staining less contrast.
Is toluidine blue a simple stain?
Toluidine Blue: A Simple, Effective Stain for Plant Tissues.
Is toluidine blue the same as methylene blue?
Conclusion. EC using toluidine blue allowed equivalent visualization of different cellular structures like methylene blue without harboring its potential side effects.
Why do neuronal bodies stain blue?
Because ammonium sulfide is a reducing agent, the stain is initially decolorized and the cell bodies appear blue only during fixation and dehydration. This makes differentiation somewhat difficult to control, and after dehydration it may be necessary to return to fixative for further differentiation.
What is metachromatic staining?
Definition of metachromatic 1 : staining or characterized by staining in a different color or shade from what is typical metachromatic granules in a bacterium. 2 : having the capacity to stain different elements of a cell or tissue in different colors or shades metachromatic stains.
What cell structure does toluidine blue stain?
Cerebellum. Toluidine blue is a basic dye that binds nucleic acids, but preferentially stains RNA. When it stains structures blue, it is called orthochromatic. At the interface between the two layers of the cerebellum , a layer of large cells known as Purkinje cells can be found.
Is methylene blue gram positive?
Often the first test performed, gram staining involves the use of crystal violet or methylene blue as the primary color. The term for organisms that retain the primary color and appear purple-brown under a microscope is Gram-positive organisms.
What is neuronal staining?
Bodian staining uses silver proteins, copper, and gold chloride to stain neuronal cell bodies (soma) and nerve processes dark brown. Normal and abnormal structures formed by abnormal fiber components are also stained.
Which parts of neurons are stained?
Dendrites, as well as the cell soma, are clearly stained in brown and black and can be followed in their entire length, which allowed neuroanatomists to track connections between neurons and to make visible the complex networking structure of many parts of the brain and spinal cord.