What kind of enzymatic activity is responsible for proofreading?
The enzyme responsible for proofreading during replication is called DNA polymerase.
What is the correct sequence of enzymes involved in DNA replication?
Helicase (unwinds the DNA double helix) Gyrase (relieves the buildup of torque during unwinding) Primase (lays down RNA primers) DNA polymerase III (main DNA synthesis enzyme)
What causes double stranded DNA breaks?
All organisms suffer double-strand breaks (DSBs) in their DNA as a result of exposure to ionizing radiation. DSBs can also form when replication forks encounter DNA lesions or repair intermediates.
What happens when DNA breaks?
The DNA in just one of your cells gets damaged tens of thousands of times per day. Because DNA provides the blueprint for the proteins your cells need to function, this damage can cause serious issues—including cancer. Fortunately, your cells have ways of fixing most of these problems, most of the time.
Which type of DNA repair is used for repairing double stranded breaks?
DNA double-strand breaks are repaired by means of two main mechanisms: nonhomologous end joining and homologous recombination (see Figure 1).
Which DNA repair process responds to single strand breaks?
Activated p53 regulates the process of DNA damage repair, the repair mechanism for single-stranded breaks is NER or BER, and they require the participation of various regulatory factors such as RPA, PCNA, p48 XP, poly ADP-ribose polymerase (PARP), X-ray repair cross-complementary protein1 (XRCC1) and DNA ligase.