Which enzyme activity is involved in proofreading during DNA replication?
Which enzyme is responsible for DNA strand replication?
What is the order of DNA replication?
Replication occurs in three major steps: the opening of the double helix and separation of the DNA strands, the priming of the template strand, and the assembly of the new DNA segment. During separation, the two strands of the DNA double helix uncoil at a specific location called the origin.
What is the difference between the leading and lagging strand in DNA replication?
Within each fork, one DNA strand, called the leading strand, is replicated continuously in the same direction as the moving fork, while the other (lagging) strand is replicated in the opposite direction in the form of short Okazaki fragments.
What is a lagging strand in DNA replication?
The lagging strand is the strand of nascent DNA whose direction of synthesis is opposite to the direction of the growing replication fork. Because of its orientation, replication of the lagging strand is more complicated as compared to that of the leading strand.
What is difference between leading and lagging strand?
The leading strand of DNA undergoes complementary base pairing smoothly as it runs in the direction from 5prime to 3prime, the same as the direction in which DNA Polymerase III works. However, the lagging strand runs in the opposite direction, from 3prime to 5prime.
How do you identify a lagging strand?
Lagging Strand:Lagging strand is a replicated strand of DNA which is formed in short segments called Okazaki fragments. DNA-ligase is required for joining Okazaki fragments.The direction of growth of the lagging strand is 3′ -» 5′ though in each Okazaki fragment it is 5′ —> 3.Starting of each Okazaki fragment requires a new RNA.