Which is brighter PE or APC?
Compared to PE, APC is not as”bright”. Compared to other red fluorophores such as Cy5, however, APC is still significantly brighter and an excellent choice for use in flow cytometry.
What is the brightest fluorophore?
It is concluded that cyanine 3.18 should be useful for multicolor fluorescence experiments and that it may be the brightest fluorophore available for single-color fluorescence immunocytochemistry.
Is af488 brighter than FITC?
Alexa Fluor 488 (Ex-Max 495 nm/Em-Max 519 nm): have nearly identical emission and excitation maxima as FITC. In addition, Alexa Flour 488 is pH insensitive (over a broad pH range) and tends to be brighter compared to FITC on most instruments.
What is PE FACS?
Phycoerythrin (PE) is one of the most commonly-used fluorescent dyes for FACS analysis. PE is a large protein (approximate molecular weight 240 kd) containing 25 fluors. Typically, only one PE molecule is conjugated to an antibody.
Is R PE the same as PE?
The protein is made up of at least three different subunits and varies according to the species of algae that produces it. The subunit structure of the most common R-PE is (αβ)6γ….Structural characteristics.
|Chromophore or other non-protein molecule||Chloride ion Cl− (CL)|
What determines fluorophore brightness?
The brightness of a fluorescent molecule depends on two factors: Extinction Coefficient (ε) – how strongly a fluorescent molecule absorbs light at a particular wavelength. Fluorescence Quantum Yield (Φ) – how efficiently the absorbed light is converted into emitted light.
What is the difference between fluorophore and fluorochrome?
As nouns the difference between fluorochrome and fluorophore is that fluorochrome is any of various fluorescent dyes used to stain biological material before microscopic examination while fluorophore is (biochemistry) a molecule or functional group which is capable of fluorescence.
Does APC and PE overlap?
Furthermore, PE (and PE-Texas Red to an even greater extent) does overlap significantly with APC.
What laser is PE excited by?
561 nm laser
PE is also excitable by the 561 nm laser and so PE based fluorophores appear on both lists. However, as PE is maximally excited at 546 nm, where possible it is advisable to use the 561 nm laser to get the maximal signal and to reduce spillover and therefore compensation.
Is Alexa Fluor 647 a tandem?
The streptavidin, Alexa Fluor™ 647-R-phycoerythrin streptavidin tandem conjugate has excitation and emission maxima at ∼496,565/667 and is widely used to detect biotinylated probes by flow cytometry in multicolor applications using a single excitation wavelength, 488 nm.
Are PE and R PE the same?
* PE is the same as R-phycoerythrin. APC, allophycocyanin; FITC, fluorescein isothiocyanate; PE, phycoerythrin; PerCP, peridinin chlorophyll protein.