Which of the following DNA sequences is a recognition site for restriction endonucleases?

Which of the following DNA sequences is a recognition site for restriction endonucleases?

palindromic sequence
A recognition sequence of a restriction endonuclease must be a palindromic sequence.

What happens at a restriction site?

A restriction site is a sequence of approximately 6–8 base pairs of DNA that binds to a given restriction enzyme. These restriction enzymes, of which there are many, have been isolated from bacteria. Their natural function is to inactivate invading viruses by cleaving the viral DNA.

Is restriction site and recognition site same?

Restriction sites, or restriction recognition sites, are located on a DNA molecule containing specific (4-8 base pairs in length) sequences of nucleotides, which are recognized by restriction enzymes.

What is the recognition site for a restriction enzyme?

Each restriction enzyme recognizes a short, specific sequence of nucleotide bases (the four basic chemical subunits of the linear double-stranded DNA molecule—adenine, cytosine, thymine, and guanine). These regions are called recognition sequences, or recognition sites, and are randomly distributed throughout the DNA.

What is a recognition site for a restriction enzyme?

Which of the following DNA sequence can be recognition site?

The correct answer is option C. BamH1 cuts just after the first Guanine from the 5’end on both the strands, giving sticky ends 5’GATCC 3′ .

What does a restriction map show?

A restriction map is a map of known restriction sites within a sequence of DNA. To find the relative positions of restriction sites on a plasmid, a technique involving single and double restriction digests is used. Based on the sizes of the resultant DNA fragments the positions of the sites can be inferred.

What are recognition sites quizlet?

Each enzyme recognises particular DNA sequences and cuts there. They are restricted where they cut. The spots where they cut are called recognition sequences. They are found in many bacterial species. Recognition sites.

What is the recognition site of a restriction enzyme?

Restriction site. These are generally palindromic sequences (because restriction enzymes usually bind as homodimers ), and a particular restriction enzyme may cut the sequence between two nucleotides within its recognition site, or somewhere nearby. For example, the common restriction enzyme EcoRI recognizes the palindromic sequence GAATTC…

What are restricted sites in DNA?

Restriction sites, or restriction recognition sites, are located on a DNA molecule containing specific sequences of nucleotides, which are recognized by restriction enzymes. These are generally palindromic sequences, and a particular restriction enzyme may cut the sequence between two nucleotides within its recognition site, or somewhere nearby.

What are restrestriction sites used for?

Restriction sites can be used for multiple applications in molecular biology such as identifying restriction fragment length polymorphisms ( RFLPs ). Several databases exist for restriction sites and enzymes, of which the largest noncommercial database is REBASE.

What is the restriction site of EcoRI?

Restriction site of EcoRI is a palindrome and it cuts DNA after G forming sticky ends with AATT. The complementary sequence of DNA for G/AATTC is CTTAA/G, where ‘/’ denotes the site where peptide bond is broken. EcoRI consists of 377 amino acids and has a molecular weight of 31kDa.