How do you make antibiotic stock?

How do you make antibiotic stock?

Preparation of 80ml stock solution

1. Tetracycline is kept in the 4C fridge in 68-564D.
2. Weigh 400mg of tetracyline HCL into a small weigh boat.
3. Dilute 95% Ethanol to 70% using milliQ water.
4. Add 80ml of 70% Ethanol to a 250ml bottle.
5. Add the tetracycline HCL to the ethanol.

How do you make a mic stock solution for antibiotics?

To prepare stock solution of 1000 µg/ml (1mg/ml) weigh 10 mg of this vancomycin hydrochloride powder with potency of 975 μg/mg and add 9.75 ml of autoclaved distilled water. This is the 1 mg/ml primary stock solution. As an alternative to avoid wastage, smaller volumes of primary stock solution can be stored and used.

How do you dilute kanamycin?

You can dilute 25 mg of kanamycin and dilute with 1 mL of dH2O and finally filter sterilized. Keep your kanamycin stock in -20C/-30C for long term use. Take 20 ul of your kanamycin stock and add with 9980 ul of sterilized dH2O. Agree with Mr.

How do you store kanamycin?

Kanamycin is light sensitive. Keep tightly closed in light-resistant containers. “Solutions are stable at 37°C for approximately 5 days. Aqueous stock solutions can be stored at 2-8°C for long term storage”, Sigma.

How do you make 10 mg/ml tetracycline?

Step 1: To prepare 10 ml of 12 mg/ml tetracycline stock solution, weigh out 120 mg of tetracycline. Transfer it to a 15 ml screw-capped tube. Add 9 ml of 70% ethanol.

What does 1000x concentration mean?

1000x concentration just means your stock is 1000x more concentrated than your working concentration. For example, if your stock is 50 mg/mL the working concentration is 50 ug/mL. To go from one to the other you dilute your stock 1000x, so for 1.5 mL you would add 1.5 uL.

Is kanamycin soluble in water?

Kanamycin sulfate is soluble in water (50 mg/ml), yielding a clear solution. Kanamycin sulfate is practically insoluble in alcohol, acetone, chloroform, ether, and ethyl acetate. A 1% solution in water has a pH of 6.5 to 8.5.

How much kanamycin do I add?

Kanamycin – add 1ml kanamycin stock (at 50mg/ml) per liter of agar to obtain a final concentration of 50ug/ml.

How long does kanamycin stock last?

Stock may be kept at -20°C for 1 year.

At what temperature does kanamycin degrade?

The results showed the reaction temperature had a significant impact on kanamycin degradation. The degradation half-life (t1/2) was shortened by 87.17-fold when the hydrothermal treatment temperature was increased from 100°C to 180°C.

How do you make tetracycline stock?

Preparation

1. Step 1: To prepare 10 ml of 12 mg/ml tetracycline stock solution, weigh out 120 mg of tetracycline. Transfer it to a 15 ml screw-capped tube. Add 9 ml of 70% ethanol. Mix until all tetracycline dissolves completely. Tip:
2. Step 2: Adjust the volume to 10 ml with 70% ethanol. Mix it again.