What color is Alexa Fluor 647?

What color is Alexa Fluor 647?

far-red
Invitrogen Alexa Fluor 647 dye is a bright, far-red–fluorescent dye with excitation ideally suited for the 594 nm or 633 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 647 dye is pH-insensitive over a wide molar range.

Is Alexa 647 Cy5?

Invitrogen Cy5 dye is a bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines. For stable signal generation, Alexa Fluor 647 dye is generally brighter than Cy5 dye in protein and nucleic acid conjugates.

What Colour is Cy5?

red
Cy3 fluoresces greenish yellow (~550 nm excitation, ~570 nm emission), while Cy5 is fluorescent in the far-red region (~650 excitation, 670 nm emission). Cy3 can be detected by various fluorometers, imagers, and microscopes with standard filters for Tetramethylrhodamine (TRITC).

What does Alexa Fluor 488?

Invitrogen Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line….Alexa Fluor 488 protein/antibody labeling kits.

Target protein Kit Cat. No.
Site-specific antibody labeling
1 mg antibody/protein Alexa Fluor 488 Antibody/Protein Labeling Kit A10235

Is Phalloidin a fluorophore?

Phalloidin, a toxin isolated from the death cap mushroom, Amanita phalloides, binds to filamentous actin with high affinity, and this has made fluorophore-conjugated phalloidin a useful tool in cellular imaging.

Is FITC a fluorochrome?

FITC (Ex-Max 494 nm/Em-Max 520 nm): Fluorescein isothiocyanate – has a very high efficiency of energy transfer from absorbed to emitted light and is one of the most commonly used fluorochromes.

What color is TRITC?

bright orange-
Tetramethylrhodamine (TRITC) is a bright orange-fluorescent dye with excitation ideally suited to the 532 nm laser line. It is commonly conjugated to antibodies and proteins for cellular imaging applications.

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