What does rpsL gene do?
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS STREPTOMYCIN RESISTANT….
| Molecular function | Ribonucleoprotein, Ribosomal protein, RNA-binding, rRNA-binding, tRNA-binding |
|---|---|
| Biological process | Antibiotic resistance |
What is the target of selection for streptomycin?
During selection at high concentrations of streptomycin the most common resistance mutations are target alteration mutations in the gene rpsL, encoding ribosomal protein S1220,21, that confer high-level resistance >1024 mg Lā122,23.
What kind of resistance mechanism do the E coli rpsL mutants have against streptomycin?
The presence of several rRNA operons (rrn) and a single rpsL gene in most bacterial genomes prohibits the isolation of streptomycin-resistant mutants in which resistance is mediated by mutations in the 16S rRNA gene (rrs).
How does the strA gene confer streptomycin resistance?
strA and strB encode phosphotransferase enzymes that modify streptomycin to a nonbactericidal form. Mutational resistance to streptomycin, caused by a point mutation-mediated target- site alteration of the ribosomal S12 protein, occurred in 1.3% of E. amylovora strains from Michigan.
What is RPSL in merchant navy?
Recruitment and Placement Services Licence (RPSL) is mandated by the Directorate General of Shipping for authorised recruiters and agents in India.
What is the mode of action for streptomycin?
Streptomycin is an aminoglycoside. It works by blocking the ability of 30S ribosomal subunits to make proteins, which results in bacterial death. Albert Schatz first isolated streptomycin in 1943 from Streptomyces griseus.
How do I get my RPSL?
Each RPS is issued with an RPSL number. A list of all registered RPSL is available on the DGS website and with us too. You must check the RPSL List before joining a shipping company. If the company is not in the list of RPSL provided by the DG Shipping, Then your joining, and the sea time will not be considered.
Is RPSL mandatory?
It is mandatory for all Recruitment and Placement Services (RPS) providers in India to register with the DGS and obtain a Recruitment and Placement Services Licence (RPSL). The full list of registered agencies can be found on the DGS website ā see here.
How do bacteria resist streptomycin?
What causes streptomycin resistance in RPSL?
Streptomycin resistance mutations in rpsL often lead to hyperaccurate but slower ribosomes. While a weak hyperaccuracy results in streptomycin resistance, a strong hyperaccuracy causes streptomycin dependence. Mutations causing streptomycin dependence have been found to map in rpsL but also in rrs (16).
What is the rpsl421 mutation?
A streptomycin -resistant strain, carrying the peculiar rpsL421 mutation, was used as a recipient for the cloning vector pNO1523, which carries the wild-type ( streptomycin -sensitive) rpsL allele [14].
What is the disease relevance of RPSL?
Disease relevance of rpsL. The rpsL gene of Escherichia coli encodes the highly conserved rps12 protein of the ribosomal accuracy centre [1]. Mutations were mapped by complementation using various transducing phages and plasmids carrying the rpsL gene, having either a normal or a defective promoter for the rpsL operon [2].
Where is the streptomycin mutation located?
The site of mutation is in the streptomycin (strA) region and is closely linked to the spcA gene [18]. This might explain why the expression of the lactose operon on rpsL strains remains coordinated when the intracellular level of formylation is decreased [19].