Why is it important to test for the PBP2a protein during the latex agglutination test?
The PBP2a test shows poor sensitivity when used directly with positive blood cultures. The gene product of mecA, an altered penicillin binding protein (PBP2a), is the hallmark of methicillin resistance in staphylococci.
Does the latex agglutination test assess presence of PBP2a?
This test is a rapid latex agglutination assay, detecting PBP2′ (also called PBP2a) 7,in isolates of Staphylococcus, as an aid in identifying methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant coagulase-negative staphylococci.
Where is staphylococcus aureus found?
Staphylococcus aureus or “staph” is a type of bacteria found on human skin, in the nose, armpit, groin, and other areas.
What is PBP2a?
PBP2a is a unique transpeptidase that is not inhibited well by β-lactam antibiotics. Hence, it is able to continue peptidoglycan crosslinking in the face of the challenge by these antibiotics, when other PBPs with transpeptidase activity are inhibited.
Why is latex agglutination testing commonly used?
Latex agglutination testing, also called latex fixation, is a diagnostic study that is widely used as a laboratory method to identify certain antibodies and antigens. The test uses a variety of body fluids including blood, urine, saliva and cerebrospinal fluid, which are dependable on what type of sample is needed.
What are the main causes of Staphylococcus aureus?
These bacteria are spread by having direct contact with an infected person, by using a contaminated object, or by inhaling infected droplets dispersed by sneezing or coughing. Skin infections are common, but the bacteria can spread through the bloodstream and infect distant organs.
How does PBP2a function in the beta lactam resistance of MRSA?
High-level resistance to β-lactam antibiotics in methicillin-resistant Staphylococcus aureus (MRSA) is due to expression of penicillin-binding protein 2a (PBP2a), a transpeptidase that catalyzes cell-wall crosslinking in the face of the challenge by β-lactam antibiotics.
Does MRSA produce PBP2a?
Methicillin-resistant Staphylococcus aureus (MRSA) produces low-affinity penicillin-binding protein 2A (PBP2A), which confers cross-resistance to most β-lactams. They are usually resistant to multiple antibiotics, leaving only vancomycin to treat severe infections (Table.
What is the role of mecA gene in MRSA?
MRSA is resistant to all β-lactams because of the presence of mecA, a gene that produces a pencillin binding protein (PBP2a) with low affinity for β-lactam antibiotics. Mechanism of oxacillin resistance other than mecA are rare.
What is Oxoid PBP2 test?
Thermo Scientific™ Oxoid PBP2′ Latex Agglutination Test is a rapid latex slide agglutination test for detection of PBP2′ as an aid in identifying methicillin resistance in both Staphylococcus aureus (MRSA) and coagulase-negative Staphylococci.
What is pbp2a7 test?
This test is a rapid latex agglutination assay, detecting PBP2/ (also called PBP2a)7, in isolates of Staphylococcus, as an aid in identifying methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant coagulase-negative staphylococci. 2. PRINCIPLES OF THE TEST
What is penicillin binding protein (PBP2)?
Penicillin-Binding Protein (PBP2/) Latex Agglutination Test 1. INTENDED USE This test is a rapid latex agglutination assay, detecting PBP2/(also called PBP2a)7, in isolates of Staphylococcus, as an aid in identifying methicillin-resistant Staphylococcus aureus(MRSA) and methicillin- resistant coagulase-negative staphylococci.
What is the difference between PBP2 positive and negative?
PBP2/ Positive (MRSA) PBP2/ Negative (MSSA) Indeterminate Strength of agglutination reaction Negative (–) = a homogeneous suspension of particles with no visible clumping Weak positive (+) = small but definite clumps against a clouded background Strong positive (+) = large and small clumps against a slightly clouded